MATERİALS AND METHODS
This study was conducted with the approval of the Istanbul Aydin
University Non-Interventional Clinical Research Ethics Committee with
decision number 2023/16. Study groups were formed based on volunteerism,
and individuals aged between 18-60 years without any chronic diseases
and immune deficiencies were selected for obese and control groups. For
obese patients, individuals with BMI ≥30 and for healthy groups,
individuals with BMI greater than 18.5 and less than 25 were selected.
10 ml of fasting blood was taken from 48 patients and 42 healthy
individuals who met these criteria. The collected blood samples were
centrifuged to separate the sera and stored at -20°C until the study was
conducted.
The seropositivity of Ad-37 and Ad-5 in the collected study samples was
investigated using ELISA test kits, which can demonstrate
antigen-antibody reactions through enzymes. The serum samples of obese
and healthy individuals were tested according to the instructions of the
test kits. Optical density measurements at a wavelength of 450 nm were
made using the Biotek-Epoch brand ELISA microplate reader.
The obtained results were interpreted as positive or negative by taking
the average of the standard samples included in the ELISA kits and
accepting it as the cutoff value. Normality analyzes were examined to
analyze the data, and it was observed that the data were not normally
distributed. Independent two-group Mann-Whitney U test, a non-parametric
analysis, was used to determine the statistical significance of the
results and the relationship between them, and a significance level of p
<0.05 was accepted.